Self-renewing Monolayer of Primary Colonic or Rectal Epithelial CellsSummary
Background & Aims: Three-dimensional organoid culture has fundamentally changed the in vitro study of intestinal biology enabling novel assays; however, its use is limited because of an inaccessible luminal compartment and challenges to data gathering in a three-dimensional hydrogel matrix.Long-lived, self-renewing 2-dimensional (2-D) tissue cultur